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laser scanning microscope zeiss lsm700  (Carl Zeiss)


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    Structured Review

    Carl Zeiss laser scanning microscope zeiss lsm700
    Laser Scanning Microscope Zeiss Lsm700, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lsm700/700+confocal+lsm+microscope/pmc12148026-141-6-5
    Average 90 stars, based on 1 article reviews
    laser scanning microscope zeiss lsm700 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Confocal Microscopy:

    Article Title: Single-cell RNA-sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS - and TARDBP -ALS
    Article Snippet: The neurons were washed twice with PBS (ThermoFisher Scientific, 70011044), followed by a single wash in ultrapure water (ThermoFisher Scientific, 10977049). .. The cells were mounted in Fluoromount G (ThermoFisher Scientific, 00-4958-02) and subjected to inverted confocal microscopy on an LSM700 (Zeiss) or LSM800-Airy microscope (Zeiss) or an Opera Phenix High Content Imaging system using the spinning disk confocal mode. .. One 10-cm dish of iPSCs was harvested by incubation with DPBS-EDTA and pelleted by centrifugation at 200 g. Cell pellets were resuspended in 400 μl RIPA buffer [Sigma Aldrich, R0278-50ML] supplemented with 2x Halt protease and phosphatase inhibitor cocktail [Thermo Fisher Scientific, 78441], 2 mM manganese sulfate, and 25 U/ml Cyanase nuclease [Serva, 18542.02] followed by incubation on ice for 30 min with vortexing in 5-min intervals.

    Microscopy:

    Article Title: Single-cell RNA-sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS - and TARDBP -ALS
    Article Snippet: The neurons were washed twice with PBS (ThermoFisher Scientific, 70011044), followed by a single wash in ultrapure water (ThermoFisher Scientific, 10977049). .. The cells were mounted in Fluoromount G (ThermoFisher Scientific, 00-4958-02) and subjected to inverted confocal microscopy on an LSM700 (Zeiss) or LSM800-Airy microscope (Zeiss) or an Opera Phenix High Content Imaging system using the spinning disk confocal mode. .. One 10-cm dish of iPSCs was harvested by incubation with DPBS-EDTA and pelleted by centrifugation at 200 g. Cell pellets were resuspended in 400 μl RIPA buffer [Sigma Aldrich, R0278-50ML] supplemented with 2x Halt protease and phosphatase inhibitor cocktail [Thermo Fisher Scientific, 78441], 2 mM manganese sulfate, and 25 U/ml Cyanase nuclease [Serva, 18542.02] followed by incubation on ice for 30 min with vortexing in 5-min intervals.

    Article Title: Pak , a downstream gene of ecdysone signaling, determines left–right polarity in the Drosophila brain through neuronal cell chirality
    Article Snippet: .. Finally, the samples were clarified with ethanol and methyl salicylate (Tokyo Chemical Industry) and observed under an LSM700 or LSM 880 microscope (Carl Zeiss). .. The following primary antibodies were used: mouse monoclonal anti-Fas2 (diluted to 1:50, DSHB), rat monoclonal anti-GFP (diluted to 1:500, Nacalai Tesque), rabbit monoclonal anti-RFP (diluted to 1:500, Nacalai Tesque), and mouse monoclonal anti-Slit (diluted to 1:50, DSHB) antibodies.

    Imaging:

    Article Title: Single-cell RNA-sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS - and TARDBP -ALS
    Article Snippet: The neurons were washed twice with PBS (ThermoFisher Scientific, 70011044), followed by a single wash in ultrapure water (ThermoFisher Scientific, 10977049). .. The cells were mounted in Fluoromount G (ThermoFisher Scientific, 00-4958-02) and subjected to inverted confocal microscopy on an LSM700 (Zeiss) or LSM800-Airy microscope (Zeiss) or an Opera Phenix High Content Imaging system using the spinning disk confocal mode. .. One 10-cm dish of iPSCs was harvested by incubation with DPBS-EDTA and pelleted by centrifugation at 200 g. Cell pellets were resuspended in 400 μl RIPA buffer [Sigma Aldrich, R0278-50ML] supplemented with 2x Halt protease and phosphatase inhibitor cocktail [Thermo Fisher Scientific, 78441], 2 mM manganese sulfate, and 25 U/ml Cyanase nuclease [Serva, 18542.02] followed by incubation on ice for 30 min with vortexing in 5-min intervals.

    other:

    Article Title: Adaptive laboratory evolution of extremophilic red microalga Cyanidioschyzon merolae under high nickel stress enhances lipid production and alleviates oxidative damage.
    Article Snippet: Cell viability of C. merolae 10NiLAD and WT cells was assessed via confocal microscopy (LSM700, Zeiss) as described by Marchetto et al. (2024), using four images per biological replicate (n = 3) and evaluating ≥ 100 cells per sample.

    Article Title: Maternal iron deficiency causes male-to-female sex reversal in mouse embryos.
    Article Snippet: Ferrous iron (Fe) is essential in all eukaryotic cells for various oxidoreductase reactions, including the demethylation of DNA and proteins.. Histone demethylation is required for normal epigenetic regulation of the Y-chromosomal sex-determining gene Sry in developing gonads during male sex determination.. Here we investigate the potential connection between iron metabolism, histone demethylation and sex determination in mammals.

    Article Title: Paraventricular hypothalamic input to anterior cingulate cortex controls food preferences in chronic visceral pain mice.
    Article Snippet: Following primary antibody incubation, the sections were incubated with the corresponding fluorophore-conjugated secondary antibodies (anti-rabbit Alexa Fluor 488, 1:500, A21206, Invitrogen and anti-mouse Alexa Fluor 555, 1:100, A31570, Invitrogen) at room temperature for 1 h. Fluorescent signals were detected using a confocal microscope (LSM700; Zeiss, Germany), processed, and exported with Zen software (Zeiss, Germany)59,60.

    Article Title: Paraventricular hypothalamic input to anterior cingulate cortex controls food preferences in chronic visceral pain mice
    Article Snippet: Following primary antibody incubation, the sections were incubated with the corresponding fluorophore-conjugated secondary antibodies (anti-rabbit Alexa Fluor 488, 1:500, A21206, Invitrogen and anti-mouse Alexa Fluor 555, 1:100, A31570 , Invitrogen) at room temperature for 1 h. Fluorescent signals were detected using a confocal microscope (LSM700; Zeiss, Germany), processed, and exported with Zen software (Zeiss, Germany) , .



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